Focus Stacking Demonstration

This is a quick focus stacking demonstration produced for a colleague after a spur-of-the-moment conversation, using whatever was under my microscope at the time. It a silicon wafer circuit, but contaminated with some microspheres floating around in some immersion oil.

A 10x lens is capable of focusing on the wafer, or on the contamination, but not both at once. The contamination is approximately 90 microns above wafer. The depth of focus of the lens is about 11 microns, making it impossible to focus on the contamination and the wafer simultaneously.

I configured the focus stacking to move over a height difference of 100 microns, ie more than ~90 required and in steps of 5 microns ie less than half the estimated depth of focus.

The video shows the microscope robotically moving 20 times in 5 micron steps capturing images and then combining them to produce an all-in-focus image with the wafer and contamination both clearly visible.

The feature was developed during Q2 of the bio project, and this demonstration video during Q3.

This is a quick focus stacking demonstration produced for a colleague after a spur-of-the-moment conversation, using whatever was under my microscope at the time. It a silicon wafer circuit, but contaminated with some microspheres floating around in some immersion oil. A 10x lens is capable of focusing on the wafer,…

This is a quick focus stacking demonstration produced for a colleague after a spur-of-the-moment conversation, using whatever was under my microscope at the time. It a silicon wafer circuit, but contaminated with some microspheres floating around in some immersion oil. A 10x lens is capable of focusing on the wafer,…